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KMID : 1149220170250020167
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Korean Journal of Oriental Medical Prescription
2017 Volume.25 No. 2 p.167 ~ p.177
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The Inhibitory Effects of GHJ on Allergic Inflammatory Response in Human Mast Cells (HMC-1)
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Hwang Man-Ki
Choe Young-Jin
Kim Min-Ju
Lee Bi-Na
Jung Hyuk-Sang
Sohn Young-Joo
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Abstract
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Objectives : This study aimed to evaluate inhibitory effects of GHJ on allergic inflammatory response in human mast cells (HMC-1).
Methods : To investigate the inhibitory effect of GHJ (62.5, 125, 250, 500, 1000 μg/mL), HMC-1 cells were stimulated with phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI). Enzyme-linked immunosorbent assays (ELISAs), RT-PCR and Western blot analysis were investigated using GHJ extract.
Results : GHJ inhibited levels of TNF-α and IL-6 of 1000 μg/mL concentration in ELISA and mRNA expression. GHJ had inhibitory effects in level of MAPKs, p-IκB-α and p-NF-kB also. GHJ attenuated Compound 48/80-stimulated histamine release. In addition, GHJ inhibited PCA reaction in vivo.
Conclusion : This study indicated that GHJ extract can inhibit allergic responses in HMC-1 cell.
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KEYWORD
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Sophora flavescens , Schizonepeta tenuifolia Briquet , Lithospermum erythrorhizom Sieb , GHJ , Allergy , HMC-1 , MAPKs , NF-kB
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